Contains ZymoBIOMICS Microbial Community Standard samples ran in different sequencing libraries as postive controls.
data(ZymoExamplePseq)Phyloseq object
Loads the data set in R.
ZymoExamplePseq contains the experimental 16S composition of ZymoBIOMICS Microbial Community Standard. 9 samples, 946 ASVs and 7 taxonomic ranks. ZymoBIOMIC mock community standards consisting of eight bacterial species Pseudomonas.aeruginosa, Escherichia coli, Salmonella.enterica, Lactobacillus.fermentum, Enterococcus.faecalis, Staphylococcus.aureus, Listeria.monocytogenes, Bacillus.subtilis and two fungal species Saccharomyces.cerevisiae and Cryptococcus.neoformans. The dilutions were done in microbial free water (Qiagen) in eight rounds of a serial three-fold dilution prior to DNA extraction. The data are for the V4 region of the 16S rRNA gene profiled with Illumina MiSeq.
Karstens L, Asquith M, Davin S, Fair D, Gregory WT, Wolfe AJ, Braun J, McWeeney S. (2019) Controlling for contaminants in low-biomass 16S rRNA gene sequencing experiments. mSystems, 4:e00290-19. https://doi.org/10.1128/mSystems.00290-19
Shetty SA 2021